• know structure, general characteristics, classification and nomenclature of enzymes;
• know molecular mechanisms and thermodynamic characteristics of enzyme act;
• know the specificity of enzyme kinetics and enzymatic processes;
• know pathways and mechanisms of enzymatic processes regulation;
• know about using enzymes in the industrial enterprise;
• know about immobilized enzymes.

Prerequisites:
General Microbiology and Virology; Biochemistry; Molecular Biology; General Biotechnology; Pharmaceutical Chemistry;
Co-requisites:
Modern Application of Biotechnological Methods; Control Methods in Biotechnology; Nanotechnologies and DNA-technologies.

Definition of enzymes, their properties, nomenclature, and classification. Enzyme structure. Groups of cofactors. Active and allosteric enzyme center. Classes of enzymes. Thermodynamic and kinetic characteristics of enzymes act. Molecular mechanisms of enzymes act. The relationship between the velocity of enzymes reaction and substrate concentration. Enzyme activity dependence on pH, temperature, concentration. Units and general principles of enzyme activity measuring. Regulation of enzymatic processes. Modifiers of enzymatic activity, activators, and inhibitors. Mechanisms of inactivation of the enzyme activity. Activation of enzymatic activity. Coenzymes and their classification. Isoenzymes. Immobilized enzymes as an area of engineering enzymology. Carriers for the immobilization of enzymes. Application of immobilized enzymes in biotechnologies, pharmaceutical, and food industry.

1. Губський Ю.І. Біологічна хімія. — Київ; Тернопіль: Укрмедкнига, 2000.
2. Герасименко В.Г. Біотехнологія: Підручник під общ. ред. В.Г. Герасименка. — К.: Фірма «ІНКОС», 2006. — 647с.
3. Иммобилизованные клетки и ферменты. Методы./ Под ред. Д. Вудворта. — М.: Мир, 1988. — 215 с.

• written report from laboratory work, oral questioning, individual research work – 40 points;
• final control (test) – 60 points (written form).