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GMO Immune and DNA Diagnostics and Identification
Major: Biotechnology and Bioengineering
Code of Subject: 8.162.00.M.22
Credits: 3
Department: Technology of Biologically Active Substances, Pharmacy and Biotechnology
Lecturer: Associate. Professor, Ph. D., Docent, Nataliya Zayarnyuk
Semester: 4 семестр
Mode of Study: денна
Learning outcomes:
• to know modern methods of molecular biotechnology and bioengineering to produce a new biotech objects;
• to know modern methods of GMO identification and immune- and DNA diagnostics;
• be able to evaluate the effects of cell and genetic modification of microorganisms; to assess risks of bioengineered products; to choose different methods of DNA analysis;
• be able to choose the technology for medicine production by molecular biotechnology methods.
• to know modern methods of GMO identification and immune- and DNA diagnostics;
• be able to evaluate the effects of cell and genetic modification of microorganisms; to assess risks of bioengineered products; to choose different methods of DNA analysis;
• be able to choose the technology for medicine production by molecular biotechnology methods.
Required prior and related subjects:
• General biotechnology
• Bioengineering (cell and genetic)
• Biotechnology of agricultural and veterinarian products
• Nanotechnologies and DNA technologies
• Biotechnology and bioengineering of plants and genome analysis methods
• Proper practice in pharmacy
• Evaluation of the quality of biopreparations and bioproduction
• Bioengineering (cell and genetic)
• Biotechnology of agricultural and veterinarian products
• Nanotechnologies and DNA technologies
• Biotechnology and bioengineering of plants and genome analysis methods
• Proper practice in pharmacy
• Evaluation of the quality of biopreparations and bioproduction
Summary of the subject:
The principles of obtaining transgenic organisms. Problems of foreign genes and proteins. The identification of GMO. Methods of immunodiagnostics, enzyme-linked immunosorbent assay, monoclonal antibodies, and hybrid cell lines. DNA diagnostics, molecular diagnostics of genetic diseases, and polymerase chain reaction, fluorescent primers. Drugs to prevent rejection of tissues and organs, drugs bound to monoclonal antibodies, HIV.
Recommended Books:
1.Електронний навчально-методичний комплекс «Технологія рекомбінантних ДНК» спец. 8.092902 „Біотехнологія біологічно активних речовин”[Електронний ресурс]. – Режим доступу: http://vns.lp.edu.ua/cours/view.php?id = 10847 Реєстраційний №E.41-218-47/2014 від 26.09.14.
2.Чепель Э. Основы клинической иммунологии / Перевод с англ. 5-е издание. — М.: ГЭОТАР-Медиа, 2008. — С. 416.
3. Глик Б., Пастернак Дж. Молекулярная биотехнология.- М.: Мир.–2002.-589с.
4.Основы молекулярной медицины / под ред. Дж. Джейсона - М.: Мир, 2002. – 764 с.
5. Патрушев Л.И. Экспрессия генов. – М.: Наука, 2007. – 652 с.
6. Якобисяк М. Імунологія. /- Вінниця.: Нова книга.-2004.
6. Уотсон, Туз, Курц. Рекомбинантные ДНК. Краткий курс. – М.: Мир, 1986. – 286 с.
2.Чепель Э. Основы клинической иммунологии / Перевод с англ. 5-е издание. — М.: ГЭОТАР-Медиа, 2008. — С. 416.
3. Глик Б., Пастернак Дж. Молекулярная биотехнология.- М.: Мир.–2002.-589с.
4.Основы молекулярной медицины / под ред. Дж. Джейсона - М.: Мир, 2002. – 764 с.
5. Патрушев Л.И. Экспрессия генов. – М.: Наука, 2007. – 652 с.
6. Якобисяк М. Імунологія. /- Вінниця.: Нова книга.-2004.
6. Уотсон, Туз, Курц. Рекомбинантные ДНК. Краткий курс. – М.: Мир, 1986. – 286 с.
Assessment methods and criteria:
• practical work and oral questioning – 30 points;
• final control (exam) – 70 points (final test – 60 points, oral test– 10 points):
• final control (exam) – 70 points (final test – 60 points, oral test– 10 points):